2.Satellie DNA is useful tool in . d)forensic science. Fusogen of protoplast culture is … coli cloning vector pBR322. B. Fusogen of protoplast culture is … FALSE. Escherichia coli cells containing plasmid pBR322 DNA were incubated with the antitumor antibiotics bleomycin A2 (BLM A2) or talisomycin A (TLM A). This is done by inserting the gene to be replicated into the plasmid, then inserting the plasmids into bacteria by a “T ”. MCQ Questions; CBSE Notes; Biology MCQs for Class 12 with Answers Chapter 11 Biotechnology: Principles and Processes. Maximum number of existing transgenic animals is of . BIOTECHNOLOGY AND ITS APPLICATION 1. Where To Download Protein Engineering Mcq Protein Engineering Mcq As recognized, adventure as capably as experience about lesson, amusement, as well as contract can be gotten by just checking out a book protein engineering mcq along with it is not directly done, you could consent even more almost this life, concerning the world. These MCQ Questions on Biotechnology: Principles and Processes Class 12 with answers pave for a quick revision of the Chapter thereby helping you to enhance subject knowledge. A. c) Rhizopus nigricans. One of the most widely used plasmids in gene cloning in bacteria is pBR322, which has both resistance genes for ampicillin and tetracycline and many restriction sites. Practice more on a regular basis with these NEET Biology objective questions on air pollution and improve your subject knowledge & problem-solving skills along with time management. For the construction of the genomic library, pBR322 was digested with EcoRI and the cohesive ends were removed and replaced by oligo(dG) molecules. 3. (d) both b and c. 40. b)organ transplantation. a)Retrovirus. A. (A) Bam HI (B) Eco RI (C) pBR322 (D) Hind III Answer :- (C) 2. plasmid: A circle of double-stranded DNA that is separate from the chromosomes, which is found in bacteria and protozoa. The main use of recombinant DNA technology are____ (a) production of transgenic humans. ‘p’ stands for ‘plasmid’, ‘B’ stands for Bolivar and ‘R’ stands for Rodriquez. Select the correct answer . 64. MCQ on Biotechnology and Genetic Engineering 1. The molecule is a double-stranded circle 4,361 base pairs in length. • Examples : pBR322 plasmid pUC18 plasmid 11. The presence of the plasmid pBR322 in cells can be detected by plating potential transformants on an agar medium that contains either (or both) of ampicillin and tetracycline antibiotics. 8. A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. Plasmids in genetic engineering • In genetic engineering, plasmids provides a versatile tool that are used to make copies of particular genes. TRUE . It contains the origin of replication(Ori) of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. Which of the following is a plasmid ? 39. Key Terms. Which one of the following is used as vector for cloning genes into higher organisms? d)Salmonella typhimurium. ; transcription: The synthesis of RNA under the direction of DNA. TRUE . Any future products from the inactivated gene will not work because of the extra codes added to it. We are here for providing you MCQ on biology topics. (a) PBR327 (b)PBR322 (c) both a above (d) None (18) Ti Plasmid is useful in (a) bringing new genes into animal cells (b) bringing new genes into plant cells (c) to nearly any sites on a chromosome (d) bringing tumour cells into plant cells (19) Many copies of a DNA molecule in a test tube are procurred by Draw a schematic sketch of pBR322 plasmid and label the following in it. When plasmids are used as cloning vectors and carry a novel DNA sequence they are referred to as chimaeric plasmids. Insertional Inactivation in pBR322 Insertional inactivation is a technique used in recombinant DNA technology and involves inactivation of a gene by inserting a fragment of DNA into the middle of its coding sequence. The plasmid pBR322 contains genes for ampicillin resistance (Ap r) and tetracycline resistance (Tc r). a) sex determination . Which of the following is a plasmid ? FIVE-CHOICE … E.coli cloning vector pBR322 showing restriction sites (Hind III, Eco Rl Bam HI, Sal I, Pvu II, Pst I, Cla I), ori and antibiotic resistance genes (amp R and tet R). (A) Bam HI (B) Eco RI (C) pBR322 (D) Hind III Answer :- (C) 2. MCQ GK; Exam Quiz; old Exams; जीवनी ; नौकरी; विशेष; search; , -Question Answer MCQ Exam ON : Gene Transfer In Plants . It is the first constructed cloning vector, created in 1977 in the laboratory of Herbert Boyer. {submitted by … Any two restriction sites. pBR322 contains the genes for resistance to ampicillin and tetracycline and can be amplified with chloramphenicol. Question: MCQ 10 Ampicillin Tetracycline PBR322 (4363 Bp) In A Molecular Cloning Experiment Using The Cloning Vector PBR322 Above, A Foreign DNA Insert Is Cloned Into The BamHl Site Of The Plasmid PBR322. EduRev is like a wikipedia just for education and the Vectors pBR322- PowerPoint Presentation, Biotechnology, Engg , Sem … (From the point of view of the aim of this experiment, … Biology MCQ. Coconut milk is used in tissue culture in which is present (a) cytokinin (b) auxin (c) gibberellin (d) ethylene. The vector was named after the researchers who constructed it for the first time. Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on … c)genetic engineering. 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